Anybody knows the exact composition of the medium for siderophores production ?

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Hariharan Balasubramaniam

Hi Wiem Alloun,

I Hope you can follow the method as described in this article for CAS medium.

Cheers,

https://www.nature.com/articles/srep32950#methods

Eugenia Iturritxa

Dear Wiem

This paper could be interesting for you.

Cheers

Article Detection of siderophore production from several fungi and b...

Aritz Burges

Hi Wiem,

I followed this very straight-forward procedure:

Article Use of Blue Agar CAS Assay for siderophore detection

which is based on the Universal siderophore assay by Schwyn & Neiland (1987):

Article Universal CAS assay for the detection and determination of s...

Achkouk Imane

Best known method for siderophores production is :Schwyn, B., Neilands, J.B., 1987. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemisty 160(1): 47-56.

Azmi Khan

Prepare CAS dye and mix with any minimal media with agar and without iron source. The solution should contain 8 parts of media with 2 parts of CAS dye.

Wiem Alloun

Thank you so much for your answers dear colleagues Azmi Khan Achkouk Imane Aritz Burges Eugenia Iturritxa Hariharan Balasubramaniam