Hi - does anyone have any advice about retrieving PBMCs from their suspension in RNAlater?

More Priya Sastry's questions See All

Requesting dataset for research purpose?

I am doing driving style analysis research with OBD data. Could anyone provide me links to get the dataset?I am not able to get it anywhere..

20 January 2021 9,707 2 View

Is a person who smokes at a higher risk of getting affected by corona virus than a non-smoker?

Is a person who smokes at a higher risk of getting affected by corona virus than that a non-smoker?

14 January 2021 4,246 30 View

Effect of the an extreme weather event on milk yield across 100 farms. Do you think the below models reflect the research question?

I have a panel data of monthly milk yield as a dependent variable and extreme weather ( in terms of percentile-based days) and other independent variables on the other side of the equation. The...

04 October 2020 8,960 1 View

How to add atomic parameters in Autodock?

How can I add atomic parameters for the ions Europium (Eu3+) and Americium (Am3+)

04 October 2020 1,718 6 View

How to modify and merge multiple chain into a single chain in Pymol?

I am looking fora command that will modify 3 chains available in the original pdb into a single chain and then renumber all of the residues. I have tried using alter command but when I export the...

26 June 2020 8,913 1 View

What difference does the UV-Visible Absorption properties undergo when a material is doped in the lattice of another nanomaterial vs nanocomposites?

I understand that when a material say, Fe is doped in say, ZnS nanoparticles, then there are no extra phases formed and there is only a shift in XRD patterns observed. But when nanocomposite is...

06 June 2020 2,255 6 View

Confirmation of dimer formation in simulation starting from two monomers?

what are the quantities that confirms formation of dimer if we simulate two monomer with specific force field

13 February 2020 283 13 View

I have created a survey for my research about leadership and change. https://www.surveymonkey.com/r/CB72MGCan you please help me by taking the survey?

https://www.surveymonkey.com/r/CB72MG6

08 February 2020 4,048 1 View

Is there a method to quantify a particular protein of interest from the crude extract?

I want to quantify a protein of ~ 7 kDa ( not a recombinant or amplified) from the crude protein extract. The sequence of the protein is known and has only one UV absorbing aminoacid.

04 February 2020 7,574 4 View

What would be a good brief food frequency questionnaire for use in parents of elementary school aged children?

I understand from the literature that child reports are more accurate than parent reports for school children, but wanted to specifically look at a brief ffq of parents for the same .

04 April 2019 8,643 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View