Hi - does anyone have any advice about retrieving PBMCs from their suspension in RNAlater?

More Priya Sastry's questions See All

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

My nanoparticle has a lower fluorescence life time of 2 ns (usual life time between 3-10 ns). what are the inferences I can get from this?

what all details we will get from fluorescence life time data

10 July 2024 505 1 View

How to troubleshoot the Milliplex Cytokine assay, when I see >OOR/<OOR in my data?

I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....

24 June 2024 9,717 0 View

How to convert mIU/mL of beta galactosidase enzyme to pg/ml? I have used ELISA to determine beta gal concentration in mIU/ mL in human serum samples?

We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?

05 June 2024 2,173 5 View

Seeking Participants for Survey on Performance-based Incentives in IT Projects, please help if possible?

Dear colleagues, I am conducting a research study on the impact of performance-based incentives on project success in the IT industry. Your participation in this survey will provide valuable...

26 May 2024 3,890 3 View

Can I apply two-step custer analysis of continuous variables for defining cases and controls?

There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...

14 May 2024 7,717 2 View

To conduct case studies among few diabetes participant?

Hi, Planning to conduct a few case studies among diabetes participants. Is there any methodology available for the same?

05 May 2024 8,371 2 View

How could i statistically find the similarities between the temperature trend (24 hrs) of two different days? what test or analysis is applicable?

I have 24-hour temperature data for 30 days at 1-hour intervals. how could I compare and find days with similar climatic trends statistically?

17 April 2024 3,211 1 View

Are you interested to collaborate with Institute of Nutrition and Fitness Sciences ?

Purpose of Collaboration - 1. Collaborative Research in the field of nutrition, health, fitness 2. Knowledge Sharing in Nutrition domains 3. Writing articles in Indexed Journals If...

14 April 2024 7,563 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View