How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View

What is the best way to filter out cell debris/small particles from a cell prep?

Hello, I am isolating lamina propria cells from intestinal tissues and I would like to add an extra step at the end to filter out cell debris but preserve my cells. Should I use a 22um filter and...

14 October 2020 3,266 2 View

T Cell Isolation for RT-PCR?

I need a cell that will be HLA-DR positive on RT-PCR. For this, would it work if I directly isolate RNA from PBMC cells with Ficoll? Or should I necessarily culture these PBMCs and isolate T cells...

12 October 2020 3,583 6 View

May somebody help me to standardise the amount and time of the enzyme mixture to isolate Cytotrophoblast from placenta??

i tried with the published protocols, but i am not getting any seperation in the ficol gradient. It seems like i am not incubating the 7gm minced tissue for proper time in the digestion...

03 October 2020 8,354 4 View

Why are there so many platelets in PBMC of mice isolated by Ficoll, and What should I do to dislodge these paltelets?

Hi, We used Ficoll to isolate PBMC from mice whole blood cells, but there are many little nights,with no celler structures, in PBMC through a microscope. We guess these granules are paltelets, but...

29 September 2020 1,667 5 View

How safely isolate RNA from blood of COVID-19 positive patients?

We are going to islotate RNA from patients with severe COVID-19 symptoms. How to inactivate the virus potentially present in the blood? We do not have equipment dedicated to work with viruses in...

20 September 2020 7,417 3 View

How to separate monocytes by plastic adhesion?

07 July 2020 5,883 10 View

Can i use ficoll or percoll to isolate lymphocytes from murine spleen?

hi every one, i want to isolate antigen-specific CTL. my plan is as follows: 1. immunize mouse with peptide twice, at 2 weeks interval; 2. after 2weeks of second immunization, kill mouse and...

15 March 2020 4,311 3 View

Can I use LS column and MidiMACS seperator from Miltenyi Biotec Neutrophil Isolation Kit for mouse peripheral blood?

We are using the neutrophil isolation kit from Miltenyi Biotec to get the neutrophils from peripheral blood from JAK2 constitutively active mice. This mouse is a leukemia model, so the traditional...

11 March 2020 8,611 3 View

What is the purpose for re-suspension after centrifugation during isolation of cells from whole blood?

I have been conducting an experiment which requires to isolate B lymphocytes from human whole blood. Several pieces of literature provide the procedure which includes re-suspension of lymphocytes...

01 February 2020 2,969 2 View