How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

How do you edit a m2p file to get nice Dssp images?

I am using the m2p file from https://ringo.ams.stonybrook.edu/index.php/MD_Simulation:_Protein_in_Water_(Pt._2) but my ss.jpg is looking pretty sad I am converting via imagemagick convert ss.eps...

17 January 2021 498 3 View

Is it possible to calculate the energy stored (kWh) in a EV battery by knowing the cathode discharge potential and specific capacity properties?

For example, if I have the following data for a LiNi0,8Co0,15Al0,05O2 compound used in the cathode: - Specific capacity = 200 mAh/g - Average voltage = 3,7 V I have no information about the...

16 January 2021 3,038 2 View

How to script VMD tcl within bash script?

Greetings, I want to run a vmd script within my bash script. I am using the text only version of VMD for linux for my windows terminal ubuntu subsystem and I would like to generate code within my...

16 January 2021 192 5 View

PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View