Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Does it mean my paper will be published if the editor encourages a full submission from me?

Hello everyone, I am new to journal paper submission. I submitted an abstract to a paper last month in reponse to a 'call for paper'. An editor came back to me a few days ago saying my abstract is...

26 November 2020 5,370 11 View

How Do You Incubate Microfluidic Chips with Cells?

Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...

21 April 2020 3,277 5 View

Is there any established relationship between angiotensin converting enzyme activity and plasmodium malarea load?

Whether malaria infected individual is at risk of developing hypertension or the inverse.

12 February 2017 9,720 2 View

How could I purify extracted genomic DNA?

Hi, in our lab we mainly work on only extracting plasmid DNA with Miniprep kit as well as purifying and receiving plasmid DNA form agarose gel. Recently I have been asked to sequence genomic DNA...

20 December 2015 7,866 2 View

How should I do samples with CFP and GFP in flow cytometry?

The CFP was suggested to be excited by the violent 405mm laser and the emission was supposed to be 450mm pacific blue but we found that AmCyan 525mm emission filter worked better. Then, the GFP...

30 August 2015 10,003 3 View

What is the mechanism of action of ursolic acid?

Anticancer drug screening.

10 May 2014 1,938 7 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

How can I change the media for THP-1 cells without dislodging them from the surface of my well plate?

I am working with THP-1 cells at the moment and am having trouble with maintaining a high confluence once I seed them on a 24-well plate. I usually try to seed around 100,000 cells per well and...

29 December 2020 1,567 3 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Does anyone have experience in infecting suspension cells (like THP-1 or K562 etc) by retrovirus?

I am trying to make stably expressing cell line of my gene of interest in Suspension cell line using retrovirus but i don't have recommended protocol. I have searched literature but mostly they...

14 December 2020 8,274 3 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

I messed up the DMSO and serial dilution standards in my MSD assay...How can I save my data?

MSD requires 8 standards that are serially diluted down in order to produce a standard curve. In the final row of my MSD Assay, I accidentally pipetted DMSO (same volume) into the last 4 wells of...

24 November 2020 3,376 2 View

Interpretation of HSV 1 and 2 Immunoflourescence ?

Hi I am M.Phil researcher in a Pathology Lab. We have purchased the SimFlour HSV 1and 2 IF kit from a reputable company. We applied it on cervical smears and cell blocks prepared from SurePath...

06 November 2020 530 1 View