Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Does it mean my paper will be published if the editor encourages a full submission from me?

Hello everyone, I am new to journal paper submission. I submitted an abstract to a paper last month in reponse to a 'call for paper'. An editor came back to me a few days ago saying my abstract is...

26 November 2020 5,370 11 View

What is the best way to present more than one research problem?

When you have three research problems is it better to write each of the problem with it's solution in same paragraph or write all the problems then the last paragraph should contain the summary of...

03 August 2020 9,805 4 View

How do i learn about MD Simulation of Asphaltene?

I want to learn about how to carry out an MD simulation of asphaltene. I have no prior knowledge, where do I start ? or is there any tool or course I can start with? Also interested in building...

13 May 2020 5,673 3 View

How Do You Incubate Microfluidic Chips with Cells?

Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...

21 April 2020 3,277 5 View

Is there any established relationship between angiotensin converting enzyme activity and plasmodium malarea load?

Whether malaria infected individual is at risk of developing hypertension or the inverse.

12 February 2017 9,720 2 View

How could I purify extracted genomic DNA?

Hi, in our lab we mainly work on only extracting plasmid DNA with Miniprep kit as well as purifying and receiving plasmid DNA form agarose gel. Recently I have been asked to sequence genomic DNA...

20 December 2015 7,866 2 View

How should I do samples with CFP and GFP in flow cytometry?

The CFP was suggested to be excited by the violent 405mm laser and the emission was supposed to be 450mm pacific blue but we found that AmCyan 525mm emission filter worked better. Then, the GFP...

30 August 2015 10,003 3 View

What is the mechanism of action of ursolic acid?

Anticancer drug screening.

10 May 2014 1,938 7 View

Can someone advise on applying discriminant analysis to partially classified data?

I have dataset that comprise of numerical and categorical scale

09 July 2013 3,747 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

If you haven't computer? how you show the photons to pupils?

Educational Process

01 March 2021 1,827 1 View