Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Does it mean my paper will be published if the editor encourages a full submission from me?

Hello everyone, I am new to journal paper submission. I submitted an abstract to a paper last month in reponse to a 'call for paper'. An editor came back to me a few days ago saying my abstract is...

26 November 2020 5,370 11 View

How Do You Incubate Microfluidic Chips with Cells?

Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...

21 April 2020 3,277 5 View

How do i go about incorporating a live classification into my built CNN?

My classification has been successful using the dataset of the tomatoes samples i captured. But now i want the classification to be done on capturing an image, making use of my built model. Your...

10 December 2019 2,788 3 View

Is there any established relationship between angiotensin converting enzyme activity and plasmodium malarea load?

Whether malaria infected individual is at risk of developing hypertension or the inverse.

12 February 2017 9,720 2 View

How could I purify extracted genomic DNA?

Hi, in our lab we mainly work on only extracting plasmid DNA with Miniprep kit as well as purifying and receiving plasmid DNA form agarose gel. Recently I have been asked to sequence genomic DNA...

20 December 2015 7,866 2 View

How should I do samples with CFP and GFP in flow cytometry?

The CFP was suggested to be excited by the violent 405mm laser and the emission was supposed to be 450mm pacific blue but we found that AmCyan 525mm emission filter worked better. Then, the GFP...

30 August 2015 10,003 3 View

What is the mechanism of action of ursolic acid?

Anticancer drug screening.

10 May 2014 1,938 7 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View