Nadra Tabassum

3 Questions 5 Answers 0 Followers

Questions related from Nadra Tabassum

What is the best incubation time for Lysis buffer with Lysozyme and Protinease inhibitor?

I'm having difficulty expressing and purifying the protein from my construct (Gateway cloning) in the pDest17 vector. The quantity is reduced after purification, with a significant background...

04 February 2022 1,663 5 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

18 February 2021 8,000 2 View

Can anyone suggest me an effective media composition and sterilization method for clonal propagation of Fern (Adiantum sp.)?

I have used Full strength of MS and Half strength of MS media along with BAP and 2,4 -D , with and without Charcol(.5mg/L/) and for sterilization I used Ethyl alcohol and HgCL2 . But no...

26 September 2016 6,184 5 View