What could be the reason for failure of ligation reaction?

03 April 2015 463 16 View

Can anyone suggest what is wrong with my PCR reaction? What is the smear in water control in PCR?

I have had good, crisp 3 bands with a set of primers. All of a sudden, these same primers are giving a smear in water control, -RT and also +RT. Internal control is fine following this and things...

03 April 2014 3,934 13 View

Why is there a variation in my PCR results?

The same set of PCR conditions including the cDNA tube once gave nice clear bands, but two days later there were absolutely no bands at all (i have repeated this twice). This pattern has occurred...

03 April 2014 4,270 5 View

What are the possible reasons for obtaining truncated PCR products with a pair of primers that are specific to the target sequence?

The template is a cDNA prepared out of a mammalian cell RNA pool.

02 March 2014 4,153 5 View

Protocol for disaggregating nanoparticle agglomerates.

I have centrifuged to pellet down the nanoparticles. Now, how can I disaggregate them into individual particles? I have tried flushing with a pastuer pippet, injection syringe and sonication (not...

01 February 2013 5,726 7 View

Absorption of nanoparticles in to the blood

I want to know whether it's possible for us to make the nanoparticles (chitosan) to get absorbed into blood escaping the digestive system.. Oral feeding is the method of nanoparticle administration.

31 December 2012 6,879 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View