Ramya Srinivasa

7 Questions 12 Answers 0 Followers

Questions related from Ramya Srinivasa

Hi, any suggestions for purifying 3KDa fluorescently tagged peptide from free dye molecules and unlabeled peptides?

this is what i have been trying to do. i have tagged N-term lysine of a synthetic 3KDa peptide with Cy3 . now i tried using 3KDa cut off centricons from millipore to remove free dye molecules....

03 March 2016 2,417 2 View

What could be the reason for failure of ligation reaction?

details: vector and insert are 2.8 Kb, both spin coloumn purified and REN checked. REN sites were introduced into insert (with 5bp extra sequences at 5`end). O/N REN digestion followed by CIP...

04 April 2015 495 16 View

Why is there a variation in my PCR results?

The same set of PCR conditions including the cDNA tube once gave nice clear bands, but two days later there were absolutely no bands at all (i have repeated this twice). This pattern has occurred...

04 April 2014 4,326 5 View

Can anyone suggest what is wrong with my PCR reaction? What is the smear in water control in PCR?

I have had good, crisp 3 bands with a set of primers. All of a sudden, these same primers are giving a smear in water control, -RT and also +RT. Internal control is fine following this and things...

04 April 2014 3,970 13 View

What are the possible reasons for obtaining truncated PCR products with a pair of primers that are specific to the target sequence?

The template is a cDNA prepared out of a mammalian cell RNA pool.

03 March 2014 4,219 5 View

Protocol for disaggregating nanoparticle agglomerates.

I have centrifuged to pellet down the nanoparticles. Now, how can I disaggregate them into individual particles? I have tried flushing with a pastuer pippet, injection syringe and sonication (not...

02 February 2013 5,763 7 View

Absorption of nanoparticles in to the blood

I want to know whether it's possible for us to make the nanoparticles (chitosan) to get absorbed into blood escaping the digestive system.. Oral feeding is the method of nanoparticle administration.

01 January 2013 6,909 9 View