might it be because of lncRNA nature? how can I explain this phenomenon?
Hi Maryam Ghanbarirad,
This is first derivatives of your melting curve, which helps you identify where are the inflection points are in the melting curve. Each peaks you see in the first derivative plot indicates a transition point. As such, you can make use of the peak center at local maximum to determine the melting temperature of the melting curve.
It seemingly suggests that a major transition point occurs at 80 degree Celsius given by the center of major peak.
As you can see, there are a few of minor melting events occurring before and following the major transition, which suggests the melting of your lncRNA is "NOT" a simple two-state unfolding process. Some "meta-stable" structural modules, rather than the most stable one, are co-existing in your lncRNA.
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