protocol for preserving RNA expression in situ
for bacteria there is this reactanct from Qiagen called RNAprotect which is a stabilizer you add to your culture before extraction, it would preserve the expresion levels and ammounts of transcript (according to them)
Hi all, Could anybody recommend for me a package or another tool that allow to combine GO terms to Heatmap of DEGs extracted from RNAseq data? Thanks
08 September 2017 7,326 0 View
I was wondering if someone could recommend the best tool for DEGs analysis? I already used the R package edgeR. Is there any difference between edgeR and DEGseq? Thanks,
05 June 2017 569 4 View
Hi all, After sorting my Bam file using Samtools, I tried to use samtools mpileup and bcftools commands for SNP calling. However, I got the following error: [E::cg] unknown type Does anybody could...
03 April 2017 1,534 2 View
I am running Trinity for my transcriptome assembly using the Cluster of our University. I got the the following error: ./runMe.sh: line 18: ../../Trinity: Permission denied. Any one could suggest...
31 December 2016 4,600 3 View
Full-length transcriptome assembly from RNA-Seq data without a reference genome
09 October 2016 8,628 2 View
R package, Raster, WorldClim database
31 December 2015 1,562 2 View
Hi,I am trying to use some softwares that require the Cornell condensed format as input! So I need to convert my data from .xls or .txt to Cornell condensed file. There some commercial...
09 October 2015 4,750 1 View
I know Patch analyst but it seems compatible only with ArcGis? GIS tools-landscape fragmentation
02 March 2015 1,347 9 View
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02 March 2013 996 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View