Package to combine GO terms to Heatmap of DEGs?

08 September 2017 7,220 0 View

RNA-seq and Differentially expressed genes?

05 June 2017 417 4 View

Samtools and BCFtools for SNP calling?

03 April 2017 1,423 2 View

Trinity, Transcriptome assembly. ./runMe.sh: line 18: ../../Trinity: Permission denied?

I am running Trinity for my transcriptome assembly using the Cluster of our University. I got the the following error: ./runMe.sh: line 18: ../../Trinity: Permission denied. Any one could suggest...

31 December 2016 4,441 3 View

Does any one could suggest for me usefull programs for full-length transcriptome assembly from RNA-Seq data without a reference genome?

Full-length transcriptome assembly from RNA-Seq data without a reference genome

09 October 2016 8,437 2 View

Can anyone tell me if there is an R package useful for extracting data from the rasters of the bioclimatic variables available in WorldClim database?

R package, Raster, WorldClim database

31 December 2015 1,423 2 View

Does anyone familiar with Cornell condensed format? Convert .txt / . xls files to Cornell condensed file?

Hi,I am trying to use some softwares that require the Cornell condensed format as input! So I need to convert my data from .xls or .txt to Cornell condensed file. There some commercial...

09 October 2015 4,629 1 View

Are there GIS tools for simulating landscape fragmentation that are compatible with QGIS?

I know Patch analyst but it seems compatible only with ArcGis? GIS tools-landscape fragmentation

02 March 2015 1,104 9 View

Does anyone have a protocol for karyotyping or determination of ploidy level of plants by using genomic DNA?

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02 March 2013 904 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View