RNA-seq and Differentially expressed genes?

05 June 2017 417 4 View

Samtools and BCFtools for SNP calling?

03 April 2017 1,423 2 View

Trinity, Transcriptome assembly. ./runMe.sh: line 18: ../../Trinity: Permission denied?

I am running Trinity for my transcriptome assembly using the Cluster of our University. I got the the following error: ./runMe.sh: line 18: ../../Trinity: Permission denied. Any one could suggest...

31 December 2016 4,441 3 View

Does any one could suggest for me usefull programs for full-length transcriptome assembly from RNA-Seq data without a reference genome?

Full-length transcriptome assembly from RNA-Seq data without a reference genome

09 October 2016 8,437 2 View

Hi all, Can anyone recommend for me an effective method/protocol for preserving the expression of Plant RNA in situ?

protocol for preserving RNA expression in situ

08 September 2016 5,370 1 View

Can anyone tell me if there is an R package useful for extracting data from the rasters of the bioclimatic variables available in WorldClim database?

R package, Raster, WorldClim database

31 December 2015 1,423 2 View

Does anyone familiar with Cornell condensed format? Convert .txt / . xls files to Cornell condensed file?

Hi,I am trying to use some softwares that require the Cornell condensed format as input! So I need to convert my data from .xls or .txt to Cornell condensed file. There some commercial...

09 October 2015 4,629 1 View

Are there GIS tools for simulating landscape fragmentation that are compatible with QGIS?

I know Patch analyst but it seems compatible only with ArcGis? GIS tools-landscape fragmentation

02 March 2015 1,104 9 View

Does anyone have a protocol for karyotyping or determination of ploidy level of plants by using genomic DNA?

.

02 March 2013 904 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View