Hemolymph extraction pupa stage?

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Is FT-IR usefull to comparte 3D printed PETG samples?

I have 2 samples I have 3D printed PETG that was put in water for 1 week and 3D printed PETG that was not put in water I want to see if some compenents leaked into the water is FT-IR usefull for...

19 May 2024 9,290 0 View

Why is hierarchical clustering the preferred clustering choice for gene expression?

Hierarchical clustering is a greedy approach to cluster a dataset following a hierarchical tree structure. This approach makes perfect sense to me for phylogenetic data to model evolutionary...

15 October 2023 2,720 2 View

Would anyone use anticoagulation after an arterial reconstruction in Whipple?

Type? (other than prophylactic LWMH) Duration?

31 August 2023 7,627 1 View

How do i measure confirmation bias (degree in which a message confirms prior beliefs) in credibility judgement of fake news messages?

Hi, I'm doing research on credibility judgement of fake news items on social media. I hypothesize that the way people judge the credibility of news messages is moderated by the degree of which the...

05 May 2021 687 2 View

Blowerdoor test with SimFlow?

For my master's thesis I am looking for someone who can help me with simflow. The research is about the reliability of airtightness tests on high buildings. We try to simulate blower door tests...

15 March 2021 3,425 0 View

Appropriate lysis buffer for immunoprecipitation?

Hi, Which lysis buffer would you recommend to make a protein extract from tissue that does not interfere with immunoprecipitation afterwards? I'm currently using RIPA + protease inhibitors,...

20 October 2019 8,981 5 View

Slower migration on SDS-page in complex sample?

Hi all, Is it possible that the migration of my protein is delayed due to the complexity of the sample? When I run a western blot with 1) a protein extract from locust tissue and 2) hemolymph...

03 June 2019 10,072 10 View

BCA assay my sample is coloured?

Hi, I've performed a protein extraction on different insect tissues using a 2D DIGE lysis buffer (containing CHAPS, ureum, thioureum and DTT). When performing a BCA assay, my samples (which often...

01 April 2019 7,570 6 View

Secondary antibody gives same signal as primary?

Hi, After immunoprecipitation and elution, I check the eluate with western blot. If I incubate the blot ONLY with secondary antibody, it gives the same signal as incubatin with primary &...

15 January 2019 4,020 4 View

Western blot on immunoprecipitation eluate detects related protein?

Hello, So, first I perform immunoprecipitation. The eluate is then subjected to immunoblot analysis using an antibody against a related protein (equal size) and gives a band. How sure am I the...

13 November 2018 5,623 7 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View