Why am I getting 2 bands of 70 KD and 55 KD for the chains of my antibodies on a SDS-PAGE gel?

Dear All, I have purified 3 variants of the monoclonal antibody from CHO cell line expressing them, using 30 KD amicon ultra-4 centrifugal filter devices following by protein G purification,...

06 July 2019 7,989 13 View

Is it practical to repeat infection process to reach better transduction efficiency?

Dear all, I'm working with lentiviral vectors and aiming to overexpress my gene of interest in CHO-k1 cell lines. I have tried several protocols to reach the high titre of the virus, however the...

04 May 2019 852 4 View

Plasmid transfection in HEK293T cell by Calsium Phosphate method ?

Dear All , we are working on gene transfection by Calcium Phosphate method but after 24h when I want to change the cell culture medium of my test and contro cells , I see that tests are not as...

11 December 2018 3,646 8 View

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

10 November 2018 7,058 5 View

What is the best protocol for lentivirus transfection in HEK293Tcells by "CaHpo4" ?

Hello everyone , I'm trying to do transfection by lentiviral vectors and my packaging cell line is HEK293T. We are going to do calcium phosphate method.I would like to find the best protocol for...

10 November 2018 3,973 4 View

Generating Stable Cell Lines with Lentivirus ?

Hello everyone We are working with HEK293T cell line. I would like to know which is the best protocol to Generating Stable Cell Lines with Lentivirus(2nd generation) ? any suggestion or comment...

09 October 2018 3,551 2 View

PEI(polyethylenimine) versus CaHpo4 and lipophectamine transfection for virus production in HEK293T cells ?

Hi, I was just wondering if anyone has any personal experience or opinions on using PEI for the production of lentivirus in 293T cells compared to calcium phosphate precipitation and...

09 October 2018 2,286 10 View

Can I use SLA1 instead of XHO1?

Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...

08 September 2018 8,697 3 View

Any suggestion or comment in regard of PCR troubleshooting ?

07 August 2018 6,772 11 View

PCR troubleshooting.what should I do to solve the problem ?

07 August 2018 4,152 7 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View