07 July 2018 1 3K Report

We used a commercial kit that extracts totall nucleic acids. You can see the electrophoresis gel in the photo.

More Niloufar Mohammadkhani's questions See All
Why am I getting 2 bands of 70 KD and 55 KD for the chains of my antibodies on a SDS-PAGE gel?

Dear All, I have purified 3 variants of the monoclonal antibody from CHO cell line expressing them, using 30 KD amicon ultra-4 centrifugal filter devices following by protein G purification,...

06 July 2019 8,171 13 View

Is it practical to repeat infection process to reach better transduction efficiency?

Dear all, I'm working with lentiviral vectors and aiming to overexpress my gene of interest in CHO-k1 cell lines. I have tried several protocols to reach the high titre of the virus, however the...

04 May 2019 978 4 View

Plasmid transfection in HEK293T cell by Calsium Phosphate method ?

Dear All , we are working on gene transfection by Calcium Phosphate method but after 24h when I want to change the cell culture medium of my test and contro cells , I see that tests are not as...

11 December 2018 3,799 8 View

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

10 November 2018 7,217 5 View

What is the best protocol for lentivirus transfection in HEK293Tcells by "CaHpo4" ?

Hello everyone , I'm trying to do transfection by lentiviral vectors and my packaging cell line is HEK293T. We are going to do calcium phosphate method.I would like to find the best protocol for...

10 November 2018 4,085 4 View

Generating Stable Cell Lines with Lentivirus ?

Hello everyone We are working with HEK293T cell line. I would like to know which is the best protocol to Generating Stable Cell Lines with Lentivirus(2nd generation) ? any suggestion or comment...

09 October 2018 3,710 2 View

PEI(polyethylenimine) versus CaHpo4 and lipophectamine transfection for virus production in HEK293T cells ?

Hi, I was just wondering if anyone has any personal experience or opinions on using PEI for the production of lentivirus in 293T cells compared to calcium phosphate precipitation and...

09 October 2018 2,421 10 View

Can I use SLA1 instead of XHO1?

Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...

08 September 2018 8,816 3 View

Any suggestion or comment in regard of PCR troubleshooting ?

Hi, Here is my PCR Reaction The primers I used were both 10µM. I added 2µl template cDNA and diluted to 10µl total with water. The cycle used was as follows: 95c

07 August 2018 6,897 11 View

PCR troubleshooting.what should I do to solve the problem ?

Hi, I recently ran a PCR Reaction using Taq 2x Master Mix RED - Ampliqon The primers I used were both 10µM. I added 2µl same template human cDNA and diluted to 10µl total with water. The cycle...

07 August 2018 4,270 7 View

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Determining the worth of a point improvement in Hamilton Depression Scale?

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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