Hi there ,
we are working on antibodies against CD20 antigen
( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 .
as far as I am concerned, Daudi , Ramos and Raji are available commercial cell lines and also a recombinant CHO cell line (ATCC).
However I don't know which one is better.
I just wonder if anyone could share their experiment, data or any article for comparison between these cases.
any comment or suggestion will be highly appreciated.
Best ,
Niloufar
Hi
Thank u for your answer but I just need comparison between those cell lines I wrote.
@HanZhang
There are a number of B-cells that are commonly used for CD20 antibody binding studies. for example Ramos or Daudi. We have used them for Rituximab (see Bondza et al. in Frontiers in Immunology, 2017. Article Real-time Characterization of Antibody Binding to Receptors ...
) but also for ofatumumab and obinutuzumab.
I have searched CD20 in my human protein database made from the PDMD (Protein-Direct-Microsequencing-Deciphering) method in vain (please see file; HepG2 Fucoidan). This may be due to famous species differences. PDMD method is a new protein identification and determination method without false-positive and false-negative.
I have found that humans express these CDs,
CD1d, CD5 antigen-like/IgM-associated peptide, CD8-alpha, CD8-beta, CD13, CD14, CD20 antigen-like 7/Membrane-spanning 4-domains subfamily A member 10, CD21, CD29, CD30, CD40, CD44, CD45, CD49b, CD49d, CD51, CD55/Complement decay-accelerating factor, CD56, CD58, CD61, CD88, CD98, CD99, CD103/Integrin alpha-E/HML-1 antigen, CD104, CD107a, CD116/Granulocyte-macrophage colony-stimulating factor receptor alpha chain/GM-CSF-R, CD117, CD127, CD129, CD152, CD154, CD163, CD233/Anion exchange protein 1/Band 3 anion transport proteinSLC4A1, CD243, CD331, CD332 (my unpublished observation).
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