16 Questions 71 Answers 0 Followers
Questions related from Niloufar Mohammadkhani
Dear All, I have purified 3 variants of the monoclonal antibody from CHO cell line expressing them, using 30 KD amicon ultra-4 centrifugal filter devices following by protein G purification,...
07 July 2019 8,135 13 View
Dear all, I'm working with lentiviral vectors and aiming to overexpress my gene of interest in CHO-k1 cell lines. I have tried several protocols to reach the high titre of the virus, however the...
05 May 2019 953 4 View
Dear All , we are working on gene transfection by Calcium Phosphate method but after 24h when I want to change the cell culture medium of my test and contro cells , I see that tests are not as...
12 December 2018 3,758 8 View
Hello everyone , I'm trying to do transfection by lentiviral vectors and my packaging cell line is HEK293T. We are going to do calcium phosphate method.I would like to find the best protocol for...
11 November 2018 4,064 4 View
Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...
11 November 2018 7,181 5 View
Hello everyone We are working with HEK293T cell line. I would like to know which is the best protocol to Generating Stable Cell Lines with Lentivirus(2nd generation) ? any suggestion or comment...
10 October 2018 3,672 2 View
Hi, I was just wondering if anyone has any personal experience or opinions on using PEI for the production of lentivirus in 293T cells compared to calcium phosphate precipitation and...
10 October 2018 2,400 10 View
Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...
09 September 2018 8,793 3 View
Hi, I recently ran a PCR Reaction using Taq 2x Master Mix RED - Ampliqon The primers I used were both 10µM. I added 2µl same template human cDNA and diluted to 10µl total with water. The cycle...
08 August 2018 4,240 7 View
Hi, Here is my PCR Reaction The primers I used were both 10µM. I added 2µl template cDNA and diluted to 10µl total with water. The cycle used was as follows: 95c
08 August 2018 6,862 11 View
I'm trying to clone my specific gene , My sample is 900bp cDNA,after pcr i run it in 1% agarose jel, 100v But I couldn't see any band of marker as well as sample. Any comment and suggestion in...
08 August 2018 5,190 4 View
we aim to extract RNA from PBMC and we use 'Jena bioscience kit', but we see only one DNA band in electrophoresis . have anyone ever worked with this kit ? What do you suggest us to solve it?
07 July 2018 304 2 View
How many PBMC can be isolated from 10 ml of blood from a healthy donor?
07 July 2018 5,348 4 View
We used a commercial kit that extracts all nucleic acids. You can see the electrophoresis gel in the photo.
07 July 2018 9,695 4 View
We used a commercial kit that extracts totall nucleic acids. You can see the electrophoresis gel in the photo.
07 July 2018 2,836 1 View
EDTA or heparin?
07 July 2018 9,619 2 View
