Dear all,
I'm working with lentiviral vectors and aiming to overexpress my gene of interest in CHO-k1 cell lines.
I have tried several protocols to reach the high titre of the virus, however the efficiency of transduction is quite low since there are very few green cells and GFP light is pale green; my crowning achievement !!
Finally I decided to check transduction repetation .I'm going to select transducted cells after antibiotic screening and infect them again. so here is my question, is this method beneficial or not?
any comment or suggestion in this regard will be highly appreciated.
Thank you in advance,