Hello.What are the best and affordable companies performing customized crispr- or shRNA/siRNA-based screens using transduced mammalian cell lines?

07 January 2020 0 10K Report

I'm considering a screen using bulk libraries (not arrayed libraries).

Badges
Science topic

More Vasco M Barreto's questions See All

Problems in measuring TG/DTG, Different results for the same sample, how to fix it?

TG measurements on the Hitachi STA7300 equipment started to have different results for the same material after some time of use Some tests have already been carried out to try to correct it: Gas...

17 May 2024 7,438 4 View

¿Qué equipo de resonancia de plasmones de superficie recomendarían y cual no para mediciones de interacciones péptido-proteína y péptido-célula?

He visto varias referencias de Biacore y todas mencionan sus ventajas. Sin embargo, nunca he usado uno, por lo que me gustaría recibir recomendaciones de personas que conozcan la técnica y que la...

16 April 2024 4,774 1 View

Free software for deconvolution of mixtures in spectroscopic data?

Is there a free software to perform simulations and to deconvolute mixtures of compounds in FORS and Raman data? I would like to: - simulate mixtures of pigments and colorants with FORS spectra -...

15 April 2024 6,393 0 View

Ways to standardize Monthly Fish catch without calculating CPUE?

I'm working with Fish catch in the Amazon, however there's no data available on the fishing vessels, fishing days or number of fishermen involved. I would like to know different approaches to...

11 April 2024 1,344 2 View

¿How do I measure the angle of a Closterium spp?

Is it measured from the apical parts to the center of the microalgae ? or do you have another ways to measured it? I attach an image to explain thanks

12 February 2024 6,924 3 View

RTgutGC cells. Does anyone know a reliable provider for these cells?

I cannot seem to find a source that sells these cells… And the papers I've read so far a not clear concerning cell line origin. It's always someone else that gave them a vial. Assinante is highly...

09 April 2023 3,159 0 View

How to calculate the Confidence Interval of a total based on the proportion of area sampled?

Hello, I've got some forestry inventory data to correct, it was not planned by me so then I got some bizarre things. I have data of the number of trees in 0.68 Ha but the total area has 1.33...

04 March 2021 6,679 4 View

How can I use ImageJ to measure the area of a Scratch assay?

I perform cell migration assays and I would like to know how to measure using ImageJ.

29 August 2020 8,169 5 View

Does anyone know how to analyse Fura-2 calcium imaging data?

I am new to calcium analysis and I have to analyse calcium imaging data of human induced pluripotent stem cell derived cardiomyocytes. Does anyone know any good tutorials/papers or have any...

10 June 2020 4,644 3 View

What is a good Selectivity Index for in vitro anticancer activity, and what paper can I cite for that value?

When we are screening extracts from natural sources, we often discard those with IC50 values higher than 20 micrograms / mL (according to Geran, R.I., Greenberg, N.H., McDonald, M.M.,...

25 May 2020 2,572 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View