How can I use ImageJ to measure the area of a Scratch assay?

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Ron Reiserer

Check out these macros - http://dev.mri.cnrs.fr/projects/imagej-macros/wiki/Wound_Healing_Tool

And this paper - Article Rapid quantification of cellular proliferation and migration...

Vinod Nadella

Check the below printed pdf link which in simple talks about how to perform scratch assay (though thats not ur current requirement) and how to analyze the images using ImageJ

https://www.le.ac.uk/biochem/microscopy/pdf/Wound%20healing%20assay.pdf

Carsten Lange

The following publications will probably help a lot:

Article Rapid quantification of cellular proliferation and migration...

Article Automated Quantification and Analysis of Cell Counting Proce...

Good luck - CL

Kumar Chokalingam

You can also try this macro for Image J

http://dev.mri.cnrs.fr/projects/imagej-macros/wiki/Wound_Healing_Coherency_Tool#:~:text=The%20Wound%20Healing%20Coherency%20Tool,images%20representing%20a%20time%2Dseries.

You can also use the Tscratch software

Nandaraj Taye

Here is a tutorial for measuring area of scratch assay using ImageJ software https://www.youtube.com/watch?v=qXzAZbL_704