RTgutGC cells. Does anyone know a reliable provider for these cells?

10 April 2023 0 3K Report

I cannot seem to find a source that sells these cells… And the papers I've read so far a not clear concerning cell line origin. It's always someone else that gave them a vial.

Assinante is highly appreciated.

Kind regards,

Vasco

Badges
Science topic

Similar topics
Correction

More Vasco Branco's questions See All

Free software for deconvolution of mixtures in spectroscopic data?

Is there a free software to perform simulations and to deconvolute mixtures of compounds in FORS and Raman data? I would like to: - simulate mixtures of pigments and colorants with FORS spectra -...

15 April 2024 6,393 0 View

Do you have access to RNA or cDNA samples positive for SARS-CoV-2?

We need a few to use as positive controls. Thank you.

17 March 2020 8,831 4 View

Hello.What are the best and affordable companies performing customized crispr- or shRNA/siRNA-based screens using transduced mammalian cell lines?

I'm considering a screen using bulk libraries (not arrayed libraries).

06 January 2020 9,584 0 View

Mammalian inducible protein expression systems: is it possible to titrate for different protein expression levels?

Hi everyone, I am looking for mammalian expression system that allows for titration dependent expression of proteins. Meaning: a system that allows me to control the level of protein expression...

16 April 2018 6,533 4 View

Sometimes reverse shoulder replacement requires glenoid bone-grafting.Is there any way to measure the glenoid bone density/predict the need for graft?

We would like to get a way to predict the need for glenoid bone-grafting in a reverse shoulder replacement planning

18 April 2017 4,339 6 View

What's the name of distal phalanx pseudarthrosis, due to interposition of ungueal matrix?

17 September 2016 8,006 3 View

At high ph (around 11), would cellulase be inhibited or denatured? Would it recovered its activity when I lower the pH again?

I am performing enzymatic saccharification using the enzyme cocktail CTec2. I am hydrolyzing alkaline pretreated lignocellulosic biomass, that I add to the reaction slowly in several steps, since...

12 September 2016 542 1 View

An increased biceps effort can result in a SLAP lesion or a tear of its tendon at the elbow. Why does it happen one or the other?

- Are there different risk factors for each one? - Why some people have one or the other lesion? - What are the main etiological differences?

14 October 2015 9,463 4 View

Which species of Freshwater Acari is this?

Hello taxonomic experts, we collected these Acari from a stream in Germany, and need some help with species identification. They all have a "Y" shaped white spot on their back. Does any one know...

01 April 2015 4,482 3 View

How can I evaluate the cell viability using the dye exclusion method (Fast Green) on adhered cells?

Can anyone please provide the simple protocol or details about the procedure of the Fast Green assay on adhered cells? All suggestions are appreciated. Thanks.

02 December 2014 9,581 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View