You can evaluate apoptosis in liver cells by several ways. Its not clear under what experimental conditions you are evaluating apoptosis in liver cells. TUNEL Assay for DNA fragmentation and Cleaved caspase-3 analysis by WB are of course convincing markers for apoptosis. The DNA ladder by agarose gel electrophoresis is considered as hall mark feature of apoptosis.
Indeed, depending on the availability of chemicals, antibodies facilities, you can explore whether death receptor pathway (FasL, intrinsic pathway) or mitochondria-mediated (extrinsic pathway; ROS-mediated) or both are operated in liver cells under certain experimental conditions. You can also go for Bax/Bcl2 ratio, P53 expression and other upstream caspases7/9 by western blotting could strengthen your findings on apoptosis.
As I said above, there are several potential markers of apoptosis, however its up to you and facilities available in your lab that can enable you to do quality grade apoptosis research in liver cells (hepatocytes).
Good Luck!
Hello Maryam Jafarsameri
Performing TUNEL assay and WB to detect the expression levels of cleaved Caspase-3 are good enough to prove/evaluate apoptosis in hepatocytes. You may also use cytochrome c.
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