Hello, please i want to know by wich component i can replace the dextrose and the Ca3 (PO4) 2 of the PVK medium (phosphate solubilisation/bacteria). Thank you
This is not my area of expertise
My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.
24 February 2021 393 14 View
Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...
18 February 2021 3,360 2 View
Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...
05 February 2021 5,523 4 View
28 December 2020 1,521 2 View
Hello, I am working on a piezoelectric energy harvester in comsol so I coupled the electrical circuit interface with the piezoelectric device multiphysics. I tried to confirm these results with...
07 December 2020 1,007 2 View
16 November 2020 4,856 8 View
Dear Colleagues, Can anyone tell me if there are Data about hormone levels in male mice , E2 Estrogen, and Testosterone blood levels in male mice. Thank you very much.
20 September 2020 1,035 3 View
opt freq gaussian calculation is performed, I got normal termination , however, the structure has nottotaly converged. because I had no convergence in Maximum Displacement! how can I solve this...
06 September 2020 4,283 3 View
i did titration to determine functional groups on the surface of activated carbon, when the titration was done on Na2HC03 and NaOH by HCl, a big volume was necessary to reach equilibrium, and...
13 July 2020 1,022 2 View
Hello, I am using a VGG16 on keras to do some image classification on a dataset, What does it mean that the validation loss is smaller all the time than the train loss?. Is 80% a reasonable...
11 July 2020 569 6 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
01 March 2021 7,254 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
22 February 2021 9,932 11 View