Hello, I would like to know what is the best method to extract DNA of Toxocara spp. eggs (for PCR application) derived from soil?

More Debbra Marcel's questions See All

Can anyone provide me with a data set suitable for discriminant analysis?

Dear all, Can anybody help me out with this? I'm looking for a dataset suitable for discriminant analysis, preferably 5 groups or less. The data are obtained with analytical chemical methods such...

17 May 2024 7,567 3 View

How to find genomic locations of genes and their exons?

Greetings everyone! For a publication I want to include analyses of pathogenic variants found in several genes (e.g. BRCA1/BRCA2) with regard to location and frequency. Thus, for the figures, I...

05 March 2024 906 4 View

Looking for citable sources on interpretation of classification metrics in the field of ML (binary criterion)?

I am currently working on a prediction-project where I am using machine learning classification techniques to do this. I have already computed various classification metrics like accuracy,...

18 September 2023 1,581 3 View

Appropriate measure and mininum sample size for inter-rater reliability with binary coding?

Dear RG community I've coded N = 500 professional development courses for teachers according to topics (0 = was not part of the course; 1 = was part of the course). I'd like to have the...

18 September 2023 2,002 1 View

How to prevent TOPAS from adding irrelevant and unnecessary bonds to CIF file? And how to handle guest molecules in voids?

I have a single crystal structure of a coordination polymer and by PXRD I can see, that I'm able to synthesize isotypical structures containing the same ligand and different metal atoms. This is...

14 September 2023 2,283 1 View

How is sustained casing pressure regulated in the middle east?

At the moment, I’m writing a paper about Sustained Casing Pressure (SCP) – pressure in gas or oil well annuli (between steel casings or between casing and tubing) that rebuilds when it’s bled down...

25 July 2023 8,890 0 View

How do I create a heatmap of passed/failed ratio in GraphPad?

Hello everyone, I have two data sets of samples that passed or failed a specific analysis. I have two variables that I would like to compare based on whether the samples passed or failed the...

12 July 2023 9,334 1 View

Are cancer cells able to metabolize medium chian fatty acids?

In the ketogenic diet MCT oils are frequently consumed. But if cancer cells are able metabolize these oils am I not putting myself at risk?

16 February 2023 9,503 10 View

Pharmacokinetics Excel model avaliable?

Does somebody has a pharmacokinetics model available in Excel? I’m working with a lot medications and would very much like to know how it builds up and to what extent. A simple model suffices but...

14 February 2023 7,359 4 View

How to increase yield of RNA of low bacterial concentration?

Hi there, I am looking for a protocol to isolate RNA using low total CFU of bacteria (S. Aureus). We already have a good isolation protocol for mammalian cells (cell culture and tissue), but now...

23 January 2023 7,018 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Arjun Verma

Hi Debbra

try CTAB method. Alot of references out there for it. USe 3-5% It works very well for sediment derived samples.

Agnieszka Paulina Kijewska

Be careful cause degradation of DNA in eggs is very fast, especially after thawing. You can use also some kits for extraction of small amounts of DNA . Something like PicoPure DNA Extraction Kit Thermoscience. It'll help you to gain good results despite the small amounts of DNA.

Ali Zamanmirabadi

You can try Chelex 100