Arjun Verma

3 Questions 17 Answers 0 Followers

Questions related from Arjun Verma

How to extract RNA from frozen filters?

Hi I am hoping to extract RNA from filter samples that have been snap frozen and been placed in -80 since. Previously I have used Trireagent plus Qiagen RNeasy kit as usual and it gives good...

04 April 2017 1,260 1 View

MOTHUR make.contigs segmentation fault?

Hi all Just received a fresh batch of Illumina MiSeq data and its giving me troubles in the very first step! I am using MOTHUR v 1.37.1 and a large number of files are showing error/segmentation...

06 August 2016 9,812 4 View

Why is RNA extraction with trireagent followed by chloroform not chloroform isoamyl?

I have been working with RNA for sometime now using Trireagent and bead beating to lyse my cells and then when it comes to the step of phase separation, I keep seeing protocols mentioning 'add...

19 November 2015 3,347 1 View