How to reconcile two sets of qPCR data from the same sample but acquired with different parameters (Ta, primer cc., etc.)?

17 November 2020 4,272 3 View

Sample size in G mellonella studies?

Hi everybody, I am working with G mellonella, and I know that one of the advantages of it is the lack of ethics commit and the 3R principles. However, I am very insecure about the sample size I...

29 October 2020 2,079 1 View

What is considered a good binding affinity energy value between macromolecules?

Hi everyone, Does exist any threshold for binding affinity value between macromolecules? E.g. Any value lower than -5.0 Kcal/Mol..... How to determine that the binding affinity among different...

22 September 2020 4,329 3 View

How to perform phase transformation modeling on ABAQUS?

Hello everyone, I am modeling an AM process called SLM, on which a laser scan a powder bed in order to build a part. I am currently having problem with the phase-transformation phenomena (from...

06 April 2017 7,623 4 View

Is there any protocol for defined media for macrophage cultures?

I want to perform an infectivity assay to test a metal compound and I need to guarantee that the medium hasn't it in a free form (apart from proteins). There is any protocol using a defined media...

19 January 2017 2,973 3 View

Should I be happy if my data gives me a null hypothesis?

I mean, if it contradicts the current literature it might be even more relevant than a positive hypothesis, but my subject in particular is already controversial. People everywhere are getting...

02 October 2016 3,052 5 View

How do I teach Inertial Reference Frame on the High School Level: ?

12 October 2015 3,551 8 View

How can I compare my infected human sample results from western blot versus ELISA?

06 July 2015 1,081 5 View

Is there any good literature for Cost-Effectiveness Analysis in Health?

Is there any good literature for Cost-Effectiveness Analysis in Health (specially oncology and Rad Onc)?Suggestion of books or articles

14 December 2014 7,645 10 View

Does anyone know an article about the influence of jasmonic acid in tuberous roots of orchids?

Review search. Just found the paper below

05 June 2014 5,915 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View