Some recent paper provides supplemental data for some cell line in the inhibition response how can I figure the sensitivity for these cell lines
1. Fast fluorescence analysis (FMCA)
Use some special fluorescent dyes (fluorescein diacetate) to stain or mark specific components of cells, or to decompose or replace non-fluorescent materials with fluorescent enzymes by fluorescent enzymes, and to measure live cells by measuring the fluorescence intensity Quantity.
Two-flow cytometer
By detecting the good linear dose-effect relationship of cells after drug action
3. Radiolabeled metabolite precursor incorporation method
Radiolabeled metabolite precursor incorporation method (3H-Tdr assay) This method uses tritium-labeled thymidine and uracil as nucleic acid metabolite precursors to determine how much-radiolabeled metabolite precursor is incorporated within a certain period of time. Judge the inhibitory effect of drugs on cell proliferation activity.
Four ATP method
Adenosine triphosphate (ATP) is the main energy source for the metabolism of living cells. When cells die, ATP hydrolyzes rapidly under the action of enzymes, so the ATP content in living cells is higher than that of dead cells. By measuring the ATP level in cancer cells, we can understand the sensitivity of anticancer drugs' degrees.
Five MTT method
Tetramethylazozole (ethyl-thiazolyl tetrazolium, MTT) can be reduced to blue-purple formazan by succinate dehydrogenase in the mitochondria of living cells. The amount of formazan formed is directly proportional to the viability of the cells.
The colorimetric method was used to determine the amount of formazan to determine the degree of drug killing on tumor cells.
That depends on what you want to find out. MTT or MTS are cheap option for anti-proliferation essay, western blot of Caspases and p53 for apoptosis evidence, cell cycle distribution is also interesting...
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