the spectrophotometer based protocol or Native PAGE based protocols are easy and simple for SOD activity see some of my papers full text available on RG for detailed protocol.

Hi Angela, we have evaluated SOD in plasma/serum by using commercial ELISA kits available from Trevigen Inc., USA. For our publications, please refer to:

1. Mishra PK, Panwar H, Bhargava A, et al. Isocyanates induces DNA damage, apoptosis, oxidative stress, and inflammation in cultured human lymphocytes. J Biochem Mol Toxicol. 2008;22(6):429-440. doi:10.1002/jbt.20260

2. Mishra PK, Khan S, Bhargava A, et al. Regulation of isocyanate-induced apoptosis, oxidative stress, and inflammation in cultured human neutrophils: isocyanate-induced neutrophils apoptosis. Cell Biol Toxicol. 2010;26(3):279-291. doi:10.1007/s10565-009-9127-9

SOD activity was measured by spectrophotometric method with minor modifications. Two reaction setups viz. experimental and reference were run in parallel. The experimental tubes contained 0.2 ml Nitro blue tetrazolium (NBT), 0.2 ml Phenozine methosulphate (PMS), 1.1 ml sodium pyrophosphate buffer and 20 µl enzyme sources, while reference tubes had all reagents except the enzyme source. Both the reactions started simultaneously after addition of 0.2 ml nicotine adenine dinucleotide, reduced (NADH). After 90 seconds, 1.0 ml glacial acetic acid was added to each tube to stop the reaction and 20 µl enzyme source was added to reference tubes. The absorbance was read at 560 nm. The difference in A560 between reference and an experimental reaction was the inhibition of NBT reduction by enzyme source. The SOD enzyme activity was defined as the amount of enzyme causing half the maximum inhibition of NBT reduction and was expressed as units/mg protein.

file:///C:/Users/Atar%20singh/Downloads/1.pdf

https://www.jbc.org/content/244/22/6049.long

Article Semecarpus anacardium nut extract promotes the antioxidant d...

Angela Dominguez you can find the method here at page 11 : Article Effect of crocin on antioxidant gene expression, fibrinolyti...

You can follow Mahmoud Hadwan methods. He has more than five methods.

Thanks for your answer!

https://www.nature.com/articles/nprot.2009.197

Western blots, activity gels and activity assays are various methods used to determine protein and activity in both cells and tissue depending on the amount of protein required for each assay. Other techniques including immunohistochemistry and immunogold can further evaluate the levels of the various antioxidant enzymes in tissues and cells.