Hello, I am using dinitrophenol and rotenone treatments on healthy fibroblasts to study cell signalling pathways (ISR and UPR). I have chosen 1mM and 500uM based on other work but have not had successful induction of these pathways. i am thinking of doing a dose response curve, in particular for DNP. How should I go about this in the most efficient way, for a pilot a experiment. I have access to 5 healthy cell lines I can use.

thanks for reading!!