Which is more accurate to calculate the IC50 : excel (dose-response curve) or through prism ?

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Abolfazl Ghoodjani

Hello Azizeh Alsayyid

Prism is clearly better.

However, you should be careful to use the right model.

Azizeh Alsayyid

Thank you for your reply. Can you please give me a guidance ( pdf or from your experience ) on which model to use. Actually we use (log (inhibitor) vs response (four parameters). However, when i used excel the Ic50 was highly variable