Hello everyone, I would like to know how to prepare a 50 mM potassium phosphate buffer with a pH of 7 and a 100 mM phosphate buffer with a pH of 7.8 ?

14 December 2023 1 3K Report

for antioxidant enzyme activity

https://www.unl.edu/cahoonlab/phosphate%20buffer.pdf

This chart should also be useful for 50 mM buffer if you just divide the volumes by 2, although the pH may be a little different.

Experimentally, you could get a pH meter and mix the monobasic and dibasic salts (100 or 50 mM each) to get the desired pH.

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