I had collected the tissues and stored in Trizol at -80°C. However, I am currently facing challenges in fully homogenizing these tissues.
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion
25 July 2024 2,486 2 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In your opinion, I would like to know if a qualitative study with a structured interview can be conducted in writing. In other words, we sent out open questions and asked the interviewees to...
11 July 2024 4,163 4 View
What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?
08 July 2024 7,464 1 View
How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...
08 July 2024 6,609 2 View
what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?
08 July 2024 7,869 1 View
In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...
26 June 2024 6,941 3 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
14 July 2024 641 1 View
I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...
23 June 2024 2,576 4 View
Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...
18 June 2024 6,854 3 View
I am extracting RNA from human adipose tissues using the TRIzol method, following the attached protocol for tissue extraction. The problem is that the 260/280 ratio of my samples is low in most...
10 June 2024 4,614 3 View
Can I store the freshly collected tissue sample in trizol instead of RNA later buffer? if Yes, How long can i store? will it affect on quality/quantity of the RNA sample? Which is better among...
02 June 2024 582 3 View
Hi, I'm a new master's student who has been studying mouse IPF model, and I read that Trizol with tissue needs to be cold. But I can't keep the tissue cold while I homogenizing it with...
30 May 2024 8,603 3 View
I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...
27 May 2024 8,025 1 View
I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I...
23 May 2024 2,475 1 View
For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...
15 May 2024 3,024 2 View