Can you help me with design of PCR amplification of long GC-rich regions?

Hi everyone, I was wondering if anyone has a solution for the amplification of long GC-regions using PCR? I want to isolate ~5kb long sequence from mouse genome that is unfortunately rich in...

24 August 2020 4,035 3 View

How to correct spherical aberation introduced by thick coverslip?

Hello I'm trying to get rid of spherical aberration in an optical tribometer, where coverslip has the form of BK7 glass disc with thickness 10 mm. Through this disc/coverslip I observing sample...

11 August 2020 7,505 4 View

Do you know about any questionnaires for children measuring their attitudes to science and scientists?

I am doing a qualitative study on children's attitudes to science and scientists before and after our science education intervention, we deliver to primary schools. I'd like to include a...

27 January 2020 8,001 3 View

Can anyone recommend a corpus of research articles written by English native speakers?

I would like to use it as a reference corpus. I know there is a SERAC corpus, but it does not seem to be available for public.

01 April 2019 5,612 6 View

Illumina NextSeq multiplexing 384 samples per run? Barcodes?

Hello, I would like to ask you if you have any experience with multiplexing of 384 samples per run on Illumina NextSeq. We are using SNP assay designed by Roche = SeqCap EZ Choice library with...

05 December 2018 4,041 3 View

Can I use Tris/HCl instead to Bis-Tris in the gradient elfo with the same working pH and MES buffer?

I try a new method for chloroplasts analysis and its my first experiment with gradient electrophoresis for membrane proteins. In the protocol is Bis-Tris used, in lab we have Tris-HCl, the working...

04 December 2017 4,831 3 View

How to report results for generalised linear mixed model with binomial distribution?

Hello, I am currently working on the data analysis for my MSc. project comparing probability of occurrence of a species between two different habitats using presence - absence data. Due to the...

29 October 2017 8,273 5 View

Mapping ChiP-seq data on HiC data?

Hello, I am a bit new to the ChiP-seq data analysis and I need to map them onto HiC data, is there any tool to do that or visualize that somehow? Either online or using R-bioconductor? Any...

22 October 2017 8,782 1 View

Any database/online dictionary showing clearly morphemes and syllables (division)?

Hello everyone. I am urgently looking for a database or dictionary that shows already fragmented words - fragmented into morphemes and syllables as well (not pronounciation). I need it for a...

16 September 2017 8,583 1 View

Characterization for human melanoma BRO cell line?

30 April 2017 8,515 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Why do we need to neutralize the charge of the system by adding ions before we proceed to energy minimization step in GROMACS ?

I am learning MD simulations using GROMACS and while I was doing a protein in water MD simulation a doubt raised. I was following the steps mentioned on the tutorials page and it said The system...

15 February 2021 5,514 4 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Anyone who used chelating agents during DNA extraction?

I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...

26 January 2021 4,542 2 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View