Hello
I'm trying to get rid of spherical aberration in an optical tribometer, where coverslip has the form of BK7 glass disc with thickness 10 mm. Through this disc/coverslip I observing sample with an infinite corrected microscope with objective lens NIKON Tu plan 20x (NA 0.4, wd 19 mm). I would like to implement 3D particle localization using astigmatism but have problems with longitudinal spherical aberration.
What are the usual ways of correcting the spherical aberration of this magnitude introduced by the disc/cover slip?
So far I was able to identify these methods after discussion with my colleagues:
- Application of adaptive optics - not sure if it is possible to correct spherical aberration of this size
- Use proprietary objective lens
- Substracting spherical aberration using measured reference
- Introducing a scattering lens in the optical path with inverse spherical aberration to the coverslip
Thanks
Dear Kryštof Dočkal,
I suggest you to see link and Attached file on subject.
https://www.zeiss.com/microscopy/int/solutions/reference/all-tutorials/basic-microscopy/coverslip-thickness-correction.html
Best regards
For thinner cover glasses there exist objective lenses with (adjustable) correction for spherical aberration. Maybe they have something also for such thick glasses?
https://www.nikon.com/products/microscope-solutions/explore/microscope-abc/learn-more-microscope/coverglass/index.htm
Adding a, most probably aspherical, compensation lens into the infinity-focused beam above the objective lens might only work properly for spots close to the axis.
You may put a plane convex lens on the cover and look through it on your sample. This should correct the spherical aberration. Try Edmund 38508 for example.
Thank you very much for the answers, I appreciate them.
Dear Henning Rehn, has the lens to be placed directly on the cover, or is there a gap like in your scheme? I don't understand how exactly your solution reduces the spherical aberration.
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