Hasan Kolkas

6 Questions 12 Answers 0 Followers

Questions related from Hasan Kolkas

Any papers to discuss why my mutants surface area got reduced after my genes of interest inactivation?

Hey all, I would like to ask if somebody can provide me with good papers to discuss the reults I got after inactivation of genes of interest in M. polymorpha. I want to discuss and relate such...

28 January 2022 9,967 0 View

How can I eliminate the non-specific bands from my PCR?

dear all, I obtained my CRISP-Cas9 mutatants last month and I am strugilling to eliminate nin-specific bands from my PCR. in fact I am gentoyping my mutants plants (haploid) using the KAPA3G PCR...

24 March 2021 6,720 9 View

Is there any protocol to extract phenolics compound prior to protein extraction?

I am trying to extract N-glycosylated proteins starting from 10 g of frozen plant lmaterial but I am facing a real problem with phenolics and oxidations. The protein solution is very dark...

07 January 2020 3,671 14 View

Is there any effiecient protocol to get rid of oxidants and polyphenols compound from my protein extract?

I havee trying to extract and purofy proteins starting from fresh plant material but always getting a lot of oxidations and polyphenols sticked to my proteins. So i would like please to ask if you...

16 December 2019 8,780 7 View

Hello everybody, i would like to ask you if there's any "free available" software that allows me to analyze my mass spectrometry excel file results?

i have got my mass spectrometry results on excel file but now i want to know how could i identify which proteins can be determined (identified) and which can't be identified? and on what criteria?...

17 November 2019 5,125 2 View

How can i avoid or eliminate oxidized molecules from my parietal protein extract?

hello all, these days i am tryingb to extract parietal proteins from Marchantia polymorpha plants but we noticed that this plant is full with oxidized molecules especially when doing protein...

22 July 2019 6,805 1 View