Hello every body, anyone know the steps for check my primers specifity (F: 5′-caatgggtgtgtttgctgtc-3′ and R: 5′-agccggtgctgtagagaaaa -3′) with PCR pro...

More Hassan Hachim Naser's questions See All

How to optimize B2L expression recombinant protein?

I have get insolube protein at 1mMIPTG after 4 hours induction time. How can I find solube protein for purification by his tag nikal colum.

09 October 2018 8,475 6 View

How to optimize B2L expression recombinant protein?

I have get insolube protein at 1mMIPTG after 4 hours induction time. I need to find insolube protein for purification in Nikal column..my protein 21kDa.

09 October 2018 5,286 2 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 4,117 20 View

Best PCR thermocycler conditions

Hi every body, i have to tried to design a groups of primers to my work about detection of some genes in bacterium (brucella spp.) i used primer3 oline software to design of my... Show moreHi...

06 July 2011 9,450 4 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 9,367 50 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 9,730 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View