How to optimize B2L expression recombinant protein?

I have get insolube protein at 1mMIPTG after 4 hours induction time. How can I find solube protein for purification by his tag nikal colum.

09 October 2018 8,382 6 View

No title

10 November 2011 1,292 3 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 4,047 20 View

Best PCR thermocycler conditions

Hi every body, i have to tried to design a groups of primers to my work about detection of some genes in bacterium (brucella spp.) i used primer3 oline software to design of my... Show moreHi...

06 July 2011 9,379 4 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 9,289 50 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 9,662 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View