How to optimize B2L expression recombinant protein?

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How to optimize B2L expression recombinant protein?

I have get insolube protein at 1mMIPTG after 4 hours induction time. How can I find solube protein for purification by his tag nikal colum.

09 October 2018 8,475 6 View

Hello every body, anyone know the steps for check my primers specifity (F: 5′-caatgggtgtgtttgctgtc-3′ and R: 5′-agccggtgctgtagagaaaa -3′) with PCR pro...

Hello every body, anyone know the steps for check my primers specifity (F: 5′-caatgggtgtgtttgctgtc-3′ and R: 5′-agccggtgctgtagagaaaa -3′) with PCR product size 233bp for housekeeping gene (glpF)...

10 November 2011 1,363 3 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 4,117 20 View

Best PCR thermocycler conditions

Hi every body, i have to tried to design a groups of primers to my work about detection of some genes in bacterium (brucella spp.) i used primer3 oline software to design of my... Show moreHi...

06 July 2011 9,450 4 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 9,367 50 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

06 July 2011 9,730 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

Omar Gonzalez-Ortega

I am having a hard time trying to understand your concern. If you want to get soluble protein you can reduce the concentration of IPTG and/or decrease temperature after induction.

Hassan Hachim Naser

Dear Dr. Thank you very much to response. I have mistake , I mean how can I get solube protein.