Maciej Zacharski

3 Questions 19 Answers 0 Followers

Questions related from Maciej Zacharski

How to reduce the haemoglobin contamination after his-tagged protein purification on cobalt resin?

Do you know any procedure to reduce the amount of haemoglobin which is co-purified with the protein of interest during cobalt affinity chromatography? We've just started to use the Promega...

07 July 2014 5,974 2 View

Where does the PCR inhibition in testicular tissue cDNA samples come from?

I've done isolation of total RNA from several testis samples, reverse transcription and in the qPCR. I got no signal from the housekeeping gene (GAPDH) in 30% of my samples; however, I got the...

09 September 2012 1,648 5 View

Real-time PCR: Where the reliability ends...

Hi! Which Ct number do you consider the border of reliability in Real-time PCR based on SYBR Green or EvaGreen? Is it 30th, 35th, 40th or 50th? Do you believe the sentence: If there is nothing to...

12 December 2011 2,274 3 View