I want to make a co-culture model with BV2 and N2a cells. For that BV2 will be upside down and I want to be sure that they don't get off the insert too easily when cultured.
When I am training deep learning model with different architectures, some time I have to change the batch size to prevent from Resource Exhausted Error condition. Is comparing the performance of...
06 April 2024 3,825 1 View
I was growing a chemical tolerant bacteria(Xanthomonas) on Nutrient agar vs Nutrient agar amended with copper. The bacteria is growing as its supposed to be on NACopper but very few or no growth...
05 November 2023 6,608 6 View
J. von Neumann: Discussion of a maximum problem. R. J. Duffin, On Fourier's Analysis of Linear Inequality Systems.
31 August 2023 5,397 4 View
I investigated the corrosion resistance PEO coated AZ91 magnesium alloy. When I compared the results from electrochemical impedance spectroscopy (EIS), namely Rp values were much higher compared...
05 June 2023 759 3 View
Any software for this purpose?
07 May 2023 5,948 2 View
Hello everyone, I'm a 2nd year student of film and media arts. I have a research paper due next week nad the task for us is to analyse a Werner Herzog documentary only using papers from Research...
15 April 2023 6,791 0 View
I want to understand how NBO (non-bridging oxygen)/T, which appears more frequently in papers on melting experiments, is calculated. I would be thankful if anybody adds a calculation spreadsheet.
06 April 2023 6,870 0 View
Hi Everyone! Richard Arthur Buckingham was the originator of the Buckingham potential formula. Although this potential is implemented in several MD codes, I haven't found online any picture about...
03 April 2023 9,280 0 View
Hello, to start some exeriments with shading I think we need to measure light quality also. Can somebody recommend some tool (all in one) which will be able to measure R/FR, UV, light quantity in...
08 March 2023 8,426 2 View
This little piece of bone has been found in the Hasle Formation of Bornholm, Denmark The formation is lower Jurassic (Pliensbachian) of age and contains a rich chondrichthyan fauna, fish, and...
06 March 2023 4,336 2 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View