Milan Boulaire

3 Questions 4 Answers 0 Followers

Questions related from Milan Boulaire

Hello, does someone know why I can't detect BDNF, NGF and TrkB mRNA expression with qPCR in Neuro-2A cells ? Does they need to be differentiated ?

I'm running a co-culture experiment with BV2 and N2a cells. I'm trying to understand how LPS treatment of BV2 can influence expression of BDNF and NGF in N2a. The problem is that I can't detect...

25 January 2023 440 6 View

I wanted to know why we use to starve cells in serum before doing a treatment (i.e. LPS, polyphenols) ?

I'm treating BV2 with vegetal compounds in order to study the effect on inflammation.

07 November 2022 4,515 1 View

Hello. Does someone can advise me on the best coating to use for BV2 cells ?

I want to make a co-culture model with BV2 and N2a cells. For that BV2 will be upside down and I want to be sure that they don't get off the insert too easily when cultured.

19 September 2022 1,043 0 View