I make a homogeneous suspension of cells before plating (~4000 cells/well).
I've tried to prepare a thin film of tungsten oxide nanoparticles on glass plate with the help of following methods- 1. Nanoparticles were dispersed in Poly-ethylene glycol (PEG) and the...
16 May 2024 9,007 1 View
Method of exogenous control standardization in miRNA Taqman assay for qPCR
24 April 2024 3,955 1 View
Instead of python or R, what tools can be used to generate heat map for sequencing data?
23 March 2024 9,100 5 View
Hi I am developing and validating a clinical interview based tool which will be administered by clinicians. To assess its qualitative face validity, I have done cognitive interviews with the...
14 January 2024 5,934 4 View
Urban bias in India’s public distribution system
31 December 2023 8,639 1 View
I have been facing the issue of False negative results in both Dengue IgG & IgM assays. I have tried various buffer compositions with commonly known blockers to resolve sample interference but...
21 December 2023 7,964 3 View
Dear all, I am developing and validating a qualitative tool using a mixed methodology. I have received feedback and ratings for the same from 6 experts in the first round of content validation,...
13 November 2023 3,548 3 View
Hi, I am developing a clinical interview tool using mixed methods, the sample size is small
31 August 2023 7,234 6 View
it would be really very helpful if someone help me in plotting this graph or give me some idea how to proceed. image reference: Yu, A. Y. C. (n.d.). ELECTRON TUNNELING AND CONTACT RESISTANCE...
24 August 2023 793 0 View
We are measuring a level of Cr that is 14% lower than our customer believes the blind sample has in it. What can be a problem like standard curves are linear, Blank count is low, and RSD is below...
21 February 2023 240 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View