Test to apply on likert scale data. I want to test the impact of e-banking on bank performance. which test should I apply?

23 October 2020 4,590 9 View

What's the problem about perovskite solar cell fabrication?

Hi, now I'm graduate student and my research part is perovskite solar cell. our research teammates started reproducibility experiment at July. and we are still doing it. however, our I-V results...

05 October 2020 5,104 11 View

Which remediation method is most suitable for heavy metals remediation from contaminated soil and groundwater nearby industrial and mining area?

Suggest me some effective and less time taking methods for remediation purpose and also economically feasible.

02 April 2019 3,186 12 View

Can I link the Ct value difference directly to the ratio of the number of transcripts?

For example, if I have Ct = 18 for GAPDH and Ct = 20 for the gene of interest, is it possible to conclude that there are 4 times more transcripts of GAPDH than transcripts of the gene of interest?

20 November 2018 8,249 4 View

Problem of urine LTE4 level in ELISA test?

14 October 2018 4,494 1 View

I am interested in knowing how to do the scoring/analysis of a sentence completion task with respect to a linguistic research question?

Is it like you provide a certain score to the different answers expected for each sentence fragment and then calculate the score later based on the response given by the participants. Please...

31 July 2017 6,537 7 View

What is the difference between many HPLC columns?

I'm trying to get conditions for my HPLC experiment! But, i'm confusing what column is fit for my HPLC condtion. i want C18 column for analyzing organic compound from my bacteria strain. i have 4...

06 July 2016 1,884 8 View

How can I fix the cell adhesion problem after subculture?

Hello, I have an suddenly and recently happened problem of HaCaT cell line after splitting step. I use the same 175T flask and 100 mm dish, fresh DMEM medium, FBS, TE buffer, but I have never...

09 May 2016 7,381 3 View

Which software is best for Degenerate primers designing?

11 February 2016 3,913 3 View

What methods can fix my results (Western blot with no band) ?

01 November 2015 3,329 10 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View