Normal flow cytometers have a dectection limid of 300 nm. Exosomes are smaller, so you can not accurately count them using standard flow cytometers such as BD FACS Canto II or FACS Calibur. You need special equipment to do so. Is there no Institute next to you that might have such a device?
Have a look at the publication from Anne Black “Platelet-derived extracellular vesicles in plateletpheresis concentrates as a quality control approach”. They used the Apogee Flow Systems.
Normal flow cytometers have a dectection limid of 300 nm. Exosomes are smaller, so you can not accurately count them using standard flow cytometers such as BD FACS Canto II or FACS Calibur. You need special equipment to do so. Is there no Institute next to you that might have such a device?
Have a look at the publication from Anne Black “Platelet-derived extracellular vesicles in plateletpheresis concentrates as a quality control approach”. They used the Apogee Flow Systems.