i want to count exosome by flowcytometry,
but i dont know which kit should i use? would you please help me in this field.
or is there any methods instead of using flowcytometry? i know NTA will help but we dont have this instrument.
thanks
Hi,
Normal flow cytometers have a dectection limid of 300 nm. Exosomes are smaller, so you can not accurately count them using standard flow cytometers such as BD FACS Canto II or FACS Calibur. You need special equipment to do so. Is there no Institute next to you that might have such a device?
Have a look at the publication from Anne Black “Platelet-derived extracellular vesicles in plateletpheresis concentrates as a quality control approach”. They used the Apogee Flow Systems.
Hi,
Normal flow cytometers have a dectection limid of 300 nm. Exosomes are smaller, so you can not accurately count them using standard flow cytometers such as BD FACS Canto II or FACS Calibur. You need special equipment to do so. Is there no Institute next to you that might have such a device?
Have a look at the publication from Anne Black “Platelet-derived extracellular vesicles in plateletpheresis concentrates as a quality control approach”. They used the Apogee Flow Systems.
Hi Gilar, I did it years agao after labeling exosomes with fluorescent-lipids and make them stick to latex beads
http://www.ncbi.nlm.nih.gov.gate2.inist.fr/pubmed/16023874
Good luck!
- Badges
- Science method