Is there any research instrument used to measure environmental sustainability as a construct?

Recommendation for an instrument used in measuring environmental sustainability empirically.

10 September 2020 282 9 View

Why my His-tagged protein cannot bind to CM-Sepharose column?

Hello, I try to purify my His-tagged protein with CM-Sepharose column, but my protein came out through the column when I washed it with 50 mM citric acid buffer without any salt. Does it mean that...

08 April 2020 8,225 6 View

How do I write a Matlab Output to Excel file?

03 November 2019 1,639 3 View

Why BSA is not added for isolation of protein for western blotting?

I am doing experiments on wheat leaves to isolate mitochondrial proteins. I have seen many references stating that we don't add BSA (in grinding and washing buffer) for isolation of proteins if we...

16 September 2018 154 3 View

How to model masonry structures in abaqus software using mohr coulomb plasticity model?

07 February 2018 2,779 1 View

What is the reason that bacteria show low solubilization index in PVK agar medium but high degree of solubilization when quantified in broth?

Is there a possibility that bacteria show low solubilization index on PVK agar medium but show quite a high solubilization efficiency in PVK broth when quantified? If so, Kindly share some literature.

07 July 2017 2,153 5 View

Can anyone suggest a facility to perform targeted sequencing of PCR products with <100k reads, > 2 X 250 bp read lenght & fast turn around time?

Both Hi-seq or Mi-seq will work for us, but we are ideally looking for something with a shorter turnaround time. As we will have only 4-5 samples at a time, it would be ideal to combine with...

31 October 2016 5,664 1 View

Optical properties with VASP?

Can anyone tell me if I can use ALGO = normal for LOPTIC calculation in VASP? The vasp example file (http://cms.mpi.univie.ac.at/wiki/index.php/Dielectric_properties_of_SiC) recomends ALGO =...

15 September 2016 1,464 1 View

Problem in calculation of optical properties with VASP?

14 September 2016 8,157 6 View

Can anyone recommend me some papers on genes involved in Phosphate solubilization and PCR primers for those gene?

For example I came to know from literature that pqq operon is very important for phosphate solubilization. Are other such genes available and provide some PCR primers for these genes

05 September 2016 4,843 2 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 8,920 1 View

How to increase titer of Herpesvirus, Adenovirus?

I'm working with Herpesvirus and Adenovirus. For Herpesvirus, I use Vero cell line to activate and check virus titer. For Adenovirus, I use Hela cell line. Recently, I could activate them, but...

16 December 2020 4,073 5 View

Why can I not find this citation about the origin of HEK-293 cells?

Hello, This citation is used on Wikipedia when explaining that the identity of the parents of the fetus from whom HEK-293 cells were taken is unknown. The link to the USFDA in the references...

07 October 2020 7,756 7 View

Do the tannic acid-treated sheep RBCs show agglutination with FAdVs (fowl adenoviruses)?

As we know that Adenoviridae indirectly agglutinates the RBCs of chicken, mice, rhesus monkey, and human blood type "O".However, do the tannic acid-treated sheep RBC show agglutination with FAdVs...

17 September 2020 6,630 3 View

How can adenovirus be inactivated in cell lysates prior to western blotting?

I need to collect cell lysates from cells treated with an adenovirus (human serotype 5) and typically use a denaturing lysis buffer such as RIPA. However, I need to inactivate any remaining...

02 September 2020 3,226 1 View

Is a recombinant adenovirus type-5 vectored COVID-19 vaccine possible?

https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(20)31208-3/fulltext

08 June 2020 2,194 4 View

Best endotoxin assay kits?

My lab routinely tests our cell lines and purified adenovirus stocks for endotoxin contamination (we consider a readout value of

01 February 2020 5,754 3 View

HEK293, expansion problems?

Recently I have been problem with HEK293a expansion problem, after 2 passages in flask, the cells stop growing and detach from flask. I am cultivating using DMEM 10 % FBS (High glucose, 10 mM...

08 September 2019 8,434 3 View

Trouble With qPCR Standard Curve Amplification and Efficiency?

I am attempting to perform a qPCR standard curve using viral DNA (Adenovirus and MCMV) and cDNA (Influenza A) to be used to determine relative abundance of these viruses in primary samples. Viral...

24 June 2019 433 4 View