I am currently working on SK-N-SH cell line, nevertheless they are growing too slow while tried both in DMEM+10%FBS and RPMI+10FBS%. I split them 1-4 and 1-2 in DMEM and RPMI accordingly but they differentiated.
If anyone is working on SK-N-SH, please let me know the best growth medium and suggestions.
thank you
Hello Suleman khan Zadran
Try using supplements in the culture medium. You can add 2mM L-glutamine and (1X) NEAA (non-essential amino acids) to the culture medium.
Culture medium: DMEM + 10% FBS or EMEM + 10% FBS.
I hope this is helpful.
Good Luck.
Read the full description on the media you are using either it is NAA+(non essential amino acids) or L glutamin + or not, if not then do supplement with these things as recommend, or if all those things were already their in your media then you can optimise the growth by splitting the cells in less no of plates. Like 1:2.
You can also check by increasing FBS/fcs-12%
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