Hello Peers,
Can anyone give me some resources or guidance on how to find the Refractive Index for Adenovirus ? Looking for any values previously reported in literature.
Thank you in advance and much appreciated!
-S
She S. A simple Google (or Google Scholar) search with 'adenovirus refractive index' provides a bunch of references. Why are you needing the RI? This will determine how many decimal places are required - for light scattering no more than 2 decimal places are adequate. Indeed for DLS no RI is needed if the intensity distribution is taken. Given that many of these biological entities are close to bags of water then it's easy to formulate that one expects an RI for 'wet'/'as is' material to be just above water and thus 1.36 - 1.37 would be a first guesstimate. Dried biological material would be higher: close to 1.50.
Alan F Rawle : Thank you for answering. Yes, for DLS. I was trying to search online but most of my searches came for AAV. Therefore, i was wondering if anyone else had any resources. How can we take account of the intensity distribution? We use the Wyatt instrument which allows us to have custom sample descriptions and RI is one of the values we can add.
She S. The default size distribution from DLS is the intensity distribution. The RI is only needed for a conversion from intensity to volume (but never, ever convert to number). Just because you can add a value doesn't mean you have to. Take a look at (free registration required):
Optical properties of organic materials
http://www.malvern.com/en/support/events-and-training/recorded-events/webinar-recorded/RW140603OpticalPropertiesOrganic.aspx
She S. You may wish to view the attached from one of my colleagues, Ulf Nobbmann
Thank you Alan F Rawle for the reference. There is also a blog on the importance that She S. may find of interest :
https://www.materials-talks.com/faq-how-important-are-refractive-index-absorption-for-nanoparticles/
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