Dear all,
Is anyone aware of any HEK reporter cell line than can be efficiently infected with HIV-1 and report an active viral transcription through a fluorescent marker downstream of an HIV-1 responsive promoter?
Dear all, This is a very specific question for the HIV biologists. Are the 'enhanced viral culture assay' and 'viral outgrowth assay' to purge latent virus synonymous? Or are they two different...
02 March 2018 9,800 0 View
Dear all, I need your feedback on the right way to analyze flow data for my experiment. I have read articles in a number of blogs and discussion forum about the usage of mean vs median...
09 October 2017 10,289 3 View
Hi all... I have a technical question here. Also, some fundamental doubts. I have tagged my protein of interest with DsRED2 RFP at the C-term end. The cloning was done taking the following...
09 October 2017 2,099 2 View
Hi all, this is a very technical query related to proximity logation assays. I am trying to locate a viral protein through proximity ligation assay. The vector containing the viral gene is...
09 October 2017 5,629 2 View
Hello all, I am trying to understand a fundamental concept in molecular biology. Is it possible that the same transcription factor might function both as an activator as well as repressor due to...
08 September 2017 7,026 4 View
I have a stable T-cell line with a human EF1alpha- ECFP cassette. Cells were selected on the basis of ECFP fluorescent marker and a low ECFP expressing population was sorted out . My question...
10 November 2014 5,201 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View