Enhanced culture assay vs Viral outgrowth assay?

Dear all, This is a very specific question for the HIV biologists. Are the 'enhanced viral culture assay' and 'viral outgrowth assay' to purge latent virus synonymous? Or are they two different...

02 March 2018 9,710 0 View

Mean vs Median vs Mode fluorescent intensity for FACS?

09 October 2017 9,913 3 View

Fluorescent protein tag confirmation by western blot?

Hi all... I have a technical question here. Also, some fundamental doubts. I have tagged my protein of interest with DsRED2 RFP at the C-term end. The cloning was done taking the following...

09 October 2017 2,027 2 View

Is Proximity ligation assay foolproof?

Hi all, this is a very technical query related to proximity logation assays. I am trying to locate a viral protein through proximity ligation assay. The vector containing the viral gene is...

09 October 2017 5,542 2 View

Opposing roles of transcription factors?

08 September 2017 6,911 4 View

Dear All, can someone tell me whether the human EF1 alpha promoter can be induced by cell activating agents like TNF alpha, PMA, PHA ?

I have a stable T-cell line with a human EF1alpha- ECFP cassette. Cells were selected on the basis of ECFP fluorescent marker and a low ECFP expressing population was sorted out . My question...

10 November 2014 5,110 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View