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Questions related from Sutanuka Chakraborty
Dear all, This is a very specific question for the HIV biologists. Are the 'enhanced viral culture assay' and 'viral outgrowth assay' to purge latent virus synonymous? Or are they two different...
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Hi all, this is a very technical query related to proximity logation assays. I am trying to locate a viral protein through proximity ligation assay. The vector containing the viral gene is...
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Hi all... I have a technical question here. Also, some fundamental doubts. I have tagged my protein of interest with DsRED2 RFP at the C-term end. The cloning was done taking the following...
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Dear all, I need your feedback on the right way to analyze flow data for my experiment. I have read articles in a number of blogs and discussion forum about the usage of mean vs median...
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Dear all, Is anyone aware of any HEK reporter cell line than can be efficiently infected with HIV-1 and report an active viral transcription through a fluorescent marker downstream of an HIV-1...
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Hello all, I am trying to understand a fundamental concept in molecular biology. Is it possible that the same transcription factor might function both as an activator as well as repressor due to...
09 September 2017 6,984 4 View
I have a stable T-cell line with a human EF1alpha- ECFP cassette. Cells were selected on the basis of ECFP fluorescent marker and a low ECFP expressing population was sorted out . My question...
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