Mean vs Median vs Mode fluorescent intensity for FACS?

09 October 2017 9,913 3 View

Fluorescent protein tag confirmation by western blot?

Hi all... I have a technical question here. Also, some fundamental doubts. I have tagged my protein of interest with DsRED2 RFP at the C-term end. The cloning was done taking the following...

09 October 2017 2,027 2 View

Is Proximity ligation assay foolproof?

Hi all, this is a very technical query related to proximity logation assays. I am trying to locate a viral protein through proximity ligation assay. The vector containing the viral gene is...

09 October 2017 5,542 2 View

HEK reporter cell line for HIV-1 study?

Dear all, Is anyone aware of any HEK reporter cell line than can be efficiently infected with HIV-1 and report an active viral transcription through a fluorescent marker downstream of an HIV-1...

08 September 2017 5,239 0 View

Opposing roles of transcription factors?

08 September 2017 6,911 4 View

Dear All, can someone tell me whether the human EF1 alpha promoter can be induced by cell activating agents like TNF alpha, PMA, PHA ?

I have a stable T-cell line with a human EF1alpha- ECFP cassette. Cells were selected on the basis of ECFP fluorescent marker and a low ECFP expressing population was sorted out . My question...

10 November 2014 5,110 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View