Heating samples when do RIPA protein extraction?

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Denis Komarov

Dear Qin,

You don't need to heat your samples before extraction, even more - working on ice is encouraged in order to preserve as much proteins as possible. Make sure not to use to much RIPA, it depends on your samples but 20-50 μL is usually enough for cells, and 50-200 to tissues. In order to get a better yield perform sonication/physical disruption (e.g. with a syringe) after collecting samples

Best of luck!