Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Desalting using DSC- 18 SPE columns?

I am looking forward to remove excess of salt form my metal based porphyrin and trying DSC-18 columns for the same, can someone suggest me how how can one decide the polarity of the solvent to...

01 February 2021 9,076 1 View

Can we use two antibodies generated in same species for indirect Sandwich ELISA?

I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...

11 January 2021 1,754 4 View

I want to quantify disturbance for wetland birds?

I have categorized the disturbance basically on movement of people and vehicle in into 3 categories. Disturbed, close to highway and movement of people from dusk to dawn. Intermediately...

30 December 2020 8,806 4 View

I am amplifying my target region through PCR, but I am getting number of unwanted band also with my target region...Why?

I have checked annealing temperature through gradient PCR. I have checked my primer in NCBI. I have checked PCR reagents concentration. Please suggest me anyone thanks

12 December 2020 1,969 2 View

What is the relationship between the type of material(ex: steel, iron etc.), thrust and torque?

If I am drilling through bars of different material(ex: iron, steel etc.) of the same thickness and under the same conditions of drill operations(feed rate and rpm), how will the torque and thrust...

06 November 2020 4,498 3 View

How to coat plasma sample (Antigen) in ELISA plate for a direct ELISA?

I have generated an antibody against an antigen. Now, I want to use the developed antibody in the detection of the unknown sample. So, I want to coat the plasma sample as antigen. How that can be...

26 October 2020 2,259 7 View

How the geospatial variability in the heat requirements of crop due to rising temperatures analyzed?

As we know that the global temperatures are increasing due to climate change which could affect the thermal needs of the crops. I want to make geospatial maps and analysis for heat requirements of...

28 September 2020 8,286 2 View

Some issues regarding electrical simulation of a Nanoowire based MSM Photodetector using Synopsys Sentaurus TCAD ?

I am simulating a NW based MSM Photodetector with Si/ CdTe/InAs NWs (modeled in SDE)with moderate doping, and have assumed a constant generation rate in the the Physics Section of SDEVICE 1....

03 September 2020 5,971 10 View

Can any body suggest the funding agency for Research Project on climate change and Remote Sensing?

I tried to submit research projects on climate change and Remote sensing to many agencies but could not get it though? Is there any easily available agencies who support the research in the subject.

16 August 2020 4,000 18 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View